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CRIMIC CR70954

Gene Hml

CG31116

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:13846054..13860001

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTCGTAAAGCGGAATAAATACGTC	GTTCTCCTTATCGACGACTTCCG

Images

Construct Design Sequences

sgRNA sequence	TATAAACAAACTGTAACTTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGGTCTTACCAGATTTTTCTTCGATAATATTGTTATATCCTTAAATTATAACAATTGAATAAACCATCTAAGTTAAAAAATGAATATATAAATAGATTTATATATACACAAAACAAACTCTCTATTAAATTGGAAATACTTTTACTTCGAAACTTTACTTTTATTAATGTCGTTGCATTAAGTATAAACAAACTGTAAC	TTAAGGAAAAAGATTTCACAACTCGCATCCATATTGAAAAAAGTGTTTTTTTTTTAAGTTGTCTTCAAAGTTACCAATACGATTCTGTAAAAATAAGTCCTTTCGCTAAAATCCTAGAAAATATTGTCCTTTCAACATTCTAATTGCTAGCTGCGTAAAATTAAGCTTTGTGTCCTAATGATTATTTGCGAATTTAGCCC

 

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