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CRIMIC CR70955

Gene Syt_alpha

CG5559

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:17592260..17604387	36D1-36D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATGAAATTGATGAGGGAGCTTGC	GTGGTATTGGACACTTGTTGCTG

Images

Construct Design Sequences

sgRNA sequence	AGCATCAGTTCGTGTTGTAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTATAGGAAAATCTTAAATAATACAAATGAAAAGAGAAAGGCTAAAACAATTCTTACTCTCAGCAAAAATGTATTTTGGTGGTGTTGCGTTTTCTTTTCTTTTATATTATTTTTCTCTTCACCCAAAGGAATACTTTAATTTGGAAAAAATTCGTTTGACAAACCTACTTAAAAGCTGTTCCAGCATCAGTTCGTGTTG	TAGGGGCACCCTTGGTGGTACTAACCTTTAAAAAATTGTTTTCGTTTCGCCCAAACAGGGAGGCTTTGGTTGCCGGTGCCGTGGGCTACAATGTGGACAACGTGAACGAGGTACCGCGTGGGAAATACAGCAGCGGAAATGCCGGTAACCTCAGCCCCACCTCTCTGAAAAGCGAGGACAATGACCCGGCCTTTTGGGTG

 

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