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CRIMIC CR70959

Gene Irk2

Irk2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:23513617..23519445	95A1-95A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAGTTGACTTCCATCATCGCC	GACACTCGCACTACTCGATTAATTGG

Images

Construct Design Sequences

sgRNA sequence	CACACTTGTCACCTGCTAAACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTTTAGCTGATTATCAGAGTGCCGCTCGCAGTTTGCGGTTGTAATTTATATGGCTACGCCCACGGTTCTAGTTTGCTTTCCGGGCTGCAGATTGCAGTGCAAATGATTTAGAGACGCTTTACGAATGCGATTCATTAGGCCAGCCATTCTGTCTTTTCCAAATCTTGACGGCATCTTGTTCTCACACTTGTCACCTGCT	AAACGGTTGTCCAAATGATTTCCCATCGGATATAGCTTCCCTTTTCCCAGACACTTTTTATTTACTATTTCGATTTTATTATTTTTTCTTATGTGTTTATTGCGTGGTCCGCTCAAAAATGTAATTAATTAATGCCGACATTCTACAAAGCGGGCCAATGATTCATATGGCACAAATTTAGCCCGCTGAGCTCTAAATTT

 

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