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CRIMIC CR70960

Gene Cp110

Cp110

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:22482082..22487458			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGATTGATGTAATGAACTCTTCAGAGG	CCTGACCAAGAATTTAAATCACCAGC

Images

Construct Design Sequences

sgRNA sequence	TATGAATCCCGGTTGCTTACGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTCCCTAGTGCCATCGTCATCTCTAATGGCAACTAAAACATAGCAGACATTTAATGAACTTTCACAAAACACCTAGATTCAGCTGACCTCTGAGAAGGCTTCCTGACATTTCGACGTCCCAACAAAAATTCCCAGTAGCAAAACACCAATGGATGCGACGTGGTGAGTGGGTCTTGGGATTTTATGAATCCCGGTTGCT	TACGGGCTTCTAATTACTGATTTTGCTCAATCTAATGCTTCTCCAATTAAGCAGGGCAATGGAGCAGCTGCTGTCTGCAGTTGAGATGGGAGGTAGCCACAGCGAGGCCACCACACCAACGGCCACGACCACCATATCGATCTGGAGCGCCCCCTCCGAGGGCAGTGGCTCTAGTTCCATGGACCCTGTCTCCTTTCGGT

 

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