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CRIMIC CR70961

Gene CG3556

CG3556

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:4530231..4535464	4C7-4C7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGGAGGATGGAGCGAGG	TGACAGGGAGAAGGACTCGG

Images

Construct Design Sequences

sgRNA sequence	TATTGCTGAGCTTAAAAATGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCAGGAACCCGACATCGGTGTGGGCCTCACGGCGGACATTATCCTCGCTTTGGGCTGGAAGGGATTGGGTGCAGTGCGGGCACTGCAGTGCGACCATGTGATACGCGAGAGCAGTGATCCTACGGGTGAGTACGGTCTCTGGGGTATCGTACATAGATTAAGCAATAGGTAAGAATGGATTAGAGAATGAATCTCCGCAT	TTTTAAGCTCAGCAATAGGGTACAGAAGTAAAGATTTGTTTCATCATTTAACGTATCTATTAAAAGATTACATTGCACTGGACTATCTATTTTCCCGCACAGAGAACGGCGAGCCAAAGCTGGCGGTGCCCTTCGGACTTAGTTCCTCTGCAGAAGAGTCGGATGCCAAGAACATCTCCTACACGTACACACTTTGGGTG

 

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