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CRIMIC CR70966

Gene nan

CG5842

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:14186249..14189648	70D3-70D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCCCATCTCGAAGCTCGTACTG	CACTCTCTCCATAATACTCGTCGC

Images

Construct Design Sequences

sgRNA sequence	GCCCAAATCGATTGTATTGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAATCGGGATCGCCCACGCACTCGCCAATTGCCCGAGATCCGAGCCCTCGAGAATCCTGACGACGATTTCAACATCCATGACTACTGCCCCGAGGTTTCGGAGGCCGAGTATATCTCCAATCCTACGGCCTATCGATTCGTCTGCTGGGATCTTAATGTGAGTGTATAATGTGGAGTGAATAAGCCCAAATCGATTGTAT	TGATGGTTTCATACTACTGCTTTTTTGGTCTATATATTTTAGAGTAAACACAGTTAATTTTAAATTTATTAATCTTTTTCAGATGCGTGGCGCTGTGGGCGAAACTATTCTCCATTTGTGCCTGCTAAATGCATCTTCCTTGCACGCAGATCTCGCCAAAAGATTGCTAAAATTCTACCCGAAACTCATCCTGGACATTT

 

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