CR70967

CRIMIC CR70967

Gene Sec61_alpha

CG9539

Information

Donor Location Cytology FBti Stock number
gRNA_int200_PM37_p1:6477182..648057526D7-26D8

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK ACACGTCGACGTCGTCAATTC TGGTACAGCAGCAATTGGG

Images

Construct Design Sequences

sgRNA sequence ATGAGCGACAAGGGTGCGAGCGG
sgRNA2 sequence
 
left_200bp right_200bp
GGCGTTCGCTGTGCAGTGAAAAGTGAGACTTTCTACTGTTCGCGTAGAAAGTGCAATAACCAAGCCACCCACTCAGTGCCCAGACTAGCAACACAAGTCCGGCAAAATGGGAAGTAAGTAACCGTCATCGCCAGACATCTTCCCCAAAATCGGGGAGTGCAGCGGTTTTTGTGTGAAGTGCCGCCCTTGCAATGCCGCTC GCACCCTTGTCGCTCATTGCTTACGTATACAAAAAAGATTCGGCGTGCGCCGCTCGTTGTGTCCGAAAATCGCAATTAATTAAAAATGGCCTGAGAAACGTAACTAATTCGGTTGCCTTAATTCACTATTTGCAGTCAAGTTCCTGGAAGTTATCAAACCGTTCTGCAGTATACTGCCGGAAATCGCAAAACCGGAGCGC
 

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