CR70967

CRIMIC CR70967

Gene Sec61_alpha

CG9539

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:6477182..6480575	26D7-26D8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ACACGTCGACGTCGTCAATTC	TGGTACAGCAGCAATTGGG

Images

Construct Design Sequences

sgRNA sequence	ATGAGCGACAAGGGTGCGAGCGG
sgRNA2 sequence

left_200bp	right_200bp
GGCGTTCGCTGTGCAGTGAAAAGTGAGACTTTCTACTGTTCGCGTAGAAAGTGCAATAACCAAGCCACCCACTCAGTGCCCAGACTAGCAACACAAGTCCGGCAAAATGGGAAGTAAGTAACCGTCATCGCCAGACATCTTCCCCAAAATCGGGGAGTGCAGCGGTTTTTGTGTGAAGTGCCGCCCTTGCAATGCCGCTC	GCACCCTTGTCGCTCATTGCTTACGTATACAAAAAAGATTCGGCGTGCGCCGCTCGTTGTGTCCGAAAATCGCAATTAATTAAAAATGGCCTGAGAAACGTAACTAATTCGGTTGCCTTAATTCACTATTTGCAGTCAAGTTCCTGGAAGTTATCAAACCGTTCTGCAGTATACTGCCGGAAATCGCAAAACCGGAGCGC