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CRIMIC CR70971

Gene Mp20

CG4696

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:13246670..13248880	49F13-49F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCAGTGTTAGCTTGTCAGAGC	GATGTTCTGACCTCGACACG

Images

Construct Design Sequences

sgRNA sequence	GCGCTTGCCGGCAATCTAGGTGG
sgRNA2 sequence	TAGAGAGCTCAAGGTTATAGAGG

 

left_200bp	right_200bp
ACCCGAAACCAAAAACCAAGAATCAAACATGTCTCTTGAGCGTGCCGTTCGTGCCAAGGTGAATACTCAATCAGTGAAGAAAAAAGATCCTTAAGAAAACATAGATATAAAGGAAAATAGATGAAAGAAATGTGAAATTACCAAACAGTTTTGTGAAATATTGTGCCTAAAATATGAATTTTTTCTTATCCGATCCACCT	TAACCTTGAGCTCTCTACGTTCTTGCTGCTGTTGTTTGTGTGGGTGTACAGACTTTATTTATCTGCCTATGGTGCGTTCTAAAAATGAATTTCATACCTATAGCCATATAGCTGAATATATATATATACTATATATGCATCATCGTATCAACGTTATAAACCAAACATAAACACACTCTGTTGAGTGTGTGCGATGTGTG

 

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