CR70974

CRIMIC CR70974

Gene Ip6k

CG10082

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:21646177..21657935	57F6-57F6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAATGCATGATGCACTTCTCG	CTCCACAAATGCTGCGATTAATC

Images

Construct Design Sequences

sgRNA sequence	GAAAGCATACTCTTCTCATCTGG
sgRNA2 sequence	GAATATATATATGGGGCAACTGG

left_200bp	right_200bp
TCACCTGAGCAGTGTTCAATTTCCATCATCCTTGAGAGTGATCGTGTTTGTCTGTTCCGAAACATTTCGTGTGTCTGCTAATTAAAACCGAGGTACCCGCGCCTCTGCCTCTGCCTCTACATTCAGCCACATCCTAATAATCCTCGTAACCCATCTTTCGTGTCGAATCGATGCTAAAATACAAACAACTACAGCCAGAT	AACTGGGAGGGAACACACAAGTTAAAAGTACAATTTGATTTCAGAATACCTAAATTGTTGTAGCAACATGAGTTAAATTATGAAACAAAATCAACTTATCTTTAGAAATTCAAAATGTGATTCAAGCAAACCTATTTAACAACCGGATATAGACCTTAACTTTTAACTTTTAACCGACGCGTAACGTTAAGTAAAAGGGC