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CRIMIC CR70976

Gene STUB1

CG5203

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:10437548..10439240	31E2-31E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCATACGATCTGTCCAAGGAGCA	TACCTTTCGACGTTCGTCAACC

Images

Construct Design Sequences

sgRNA sequence	AATATATATTCTATCACATGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACTTTGGGGATGATATTACACTACAGTTGCGACTAGCTCGCAAAAAGCGCTGGAATGTTATGGAGGAGAAGCGAATACAGCAGGAAATCGAGCTGCAAAGCTATTTAAATGGGTAAGATGTTATTCGAATTCTCTAGATGCCTTTTGCAAACTAAATTTAGTCACCGCACGACGATATCGAGGCAATTGTTGTCCTCAT	GTGATAGAATATATATTAAAAAAAGCGAAATTTGTGCAAAACCTAAATATTTATGTTTCAGTCTAATAAAAGGGGACATGGAAAGCCGTTTGGCCAATTTAAAGCTGAATGGAAATGTACACGATGAGCAGCTGAAAGACAAGCAACAGGAAATTGAGCAAGAATGCGTAAGTTGGACTTTAACCAGAATATTTAGCGGA

 

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