CR70977

CRIMIC CR70977

Gene fz4

CG4626

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:7106272..7110321	6F5-6F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAAGAAGGAGAGCTGGAAGTTG	GATGACCTATGACCTCGCGATAAAG

Images

Construct Design Sequences

sgRNA sequence	ATTCGATACGTTTCTTCGCTTGG
sgRNA2 sequence	ATTAGGTACCATTTCAGGGCAGG

left_200bp	right_200bp
AGAGGGTGCGAGCGAGAGAGGGCGAACGCACCTGGAACAGGTTACCCCTTCCAGCGTTCGTTCCACCGAAGAACGAAGAATCTGCATATAGTTCGGATTCTTTGGAAAGAAAAGAGGGAACCCAGCGCGAAGAAAACCCCGAAATCTGATCTTTTCCACATTTCGATTCCTTTGGAGAAACGGAAATCGGAACACCAAGC	GGCAGGAAATGCTTTCTGCTTCCAGTCAATGTTCCTGCAGCAGCTGCTGTGCCAAAGTCAATCCTCTACTATAACTTAATGCGGTTTCGGGTATCGAAAGAGGCAAAGCAACTTAAATGGGTGGGGAAACGGTCCAATTTGTTTGTCATAAAACGATCGTGAGATAACGATATCGTTATTTCGATTTTAGTTGCTTTGCC