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CRIMIC CR70979

Gene Rnf146

CG8786

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:19637207..19622816	76B11-76B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCTGGAGCTGCTGCTTC	GCAGGTTTGCAAACAGATGGG

Images

Construct Design Sequences

sgRNA sequence	CATTTCAAAGCAATACTAGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGCCTGACGAAGGAGCGTTAGCCACCGGTGTGGATGCAGGTAAGCAAAGTTGCCTTTCGCCACAAGAAGTTCCCTTTGCTTTTATAAAGCTTTAGGATACACTTGCACTATTCCTAGAGCGCATACACACGCATATGTACATATACATATGTACGAAATCAAATTTGAAGGACTTCCTAAAAGCATTTCAAAGCAATACT	AGAAGGAATTATGAATTATGACAGATTATTTTGCACTCACTAGCACAATAATATTCTATAATATTATTGAGAGCACAAGAGTTATACTTTTGCATCGTTATTATTCATATATCCCTTAATCCTTATTCTATTTGATTTAACTTATTTCAATTAACTCAATTTCTAGCGGCAGCCGTTTCTGGCGAAGATTCCCCCTCTGC

 

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