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CRIMIC CR70980

Gene slou

CG6534

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:21548661..21557182	93E4-93E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGCACTAAGTGCAAGGATTTCG	CCTTAATCACCGACTGGTAAACTAC

Images

Construct Design Sequences

sgRNA sequence	TTTCTCGACCAAGTGTTAATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGATTTATTAAAATACTGGTTCTAAAATGAAGGCTGTTAGTGAAAATTTGAAAACTTAAATAGCAAATTTATTATTAATGTTGATATTTATTGCCCACACAATTGGTAGCTTTTATTAGCGTTGGCTAATACAAATTGATTTTATGTTCTGCTCAATTAGCTTTTTTATTAGATTTTGCGTTCGAGTAATTAACCCAATT	AACACTTGGTCGAGAAAATATGCCAAAATTAACCTTTTTGTATGTTTATAGCTCGATTTTGAGATTTTCATTTTGTTTTTAAGCGTGTGGTATGTTGAGGAATTTGATTTATATCTATAGATTTTTATTTATAATTGGTATACTTTTCCAAATATATTTTTTATATTTTAGTTTAACTAAATTATATCACCTAGGACAAC

 

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