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CRIMIC CR70983

Gene ktub

CG9398

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:21101928..21123704	57B20-57C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAGTTGCTGTACCGACAG	GACCAACCTTCCCAACTTAATCG

Images

Construct Design Sequences

sgRNA sequence	GCAACTTCCTCCATCCCTTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCATAATTGCAAGTGAATTAGCAAATCCCTCCGGCGGGCCATGTGGTTGAGTCCATTGAGTCACTTTCCTTCTCTGTCCATGAATATTCAATGCGACGACAGACCTTATTCATTGCCATTCAATGTGTTCAATATGTGCTGAAAGTGGTGCAGAATCCTACGCCTCATCTCCTCCTTCCGCGGCAACTTCCTCCATCCC	TTGTGGCTCGTTGATGGCCCCCATCCGTCCGGTTCCGCCGCCGCCGCCGGACTTGGTAAGTTTCCCCCGTCATCGCATTCGGATTGCCATTTTCCAGTTACCGCAATCAGAGGCGCACACAGGGCGTTTATATTGAAAGTTGCCAATTGGCTGGAAAATGCCAGAGAAGATTTTCAACAGTACCCCGGTTTACTGTTCAC

 

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