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CRIMIC CR70985

Gene Tsp5D

CG4690

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:6069131..6074857	5D3-5D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GACAGCATGTTGCATTGTCTGAGC	ACGCACAGCTACATAACTGC

Images

Construct Design Sequences

sgRNA sequence	CTGAAGGATCTATCATTCAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGACTTTGGCGGCGTGTTTACCCTTAGCTTTTTGGAGCTGCAGCTGCAGCTTCCGTCCGCTTTTGTTTTTGCTTGCCACTGGGTTTCCCCCGAAGTCCCTGAAAATGTCAGATGTCAGGCCGTTGTGAACATGCAACAGTTTGCAATCGAAAGCTCTCCATTTGGCCGTCAATCGGGGGAATTATCTGATTTCGCCTCTG	AATGATAGATCCTTCAGCTATTCGTGCAGCAAAGAGCAATAAATCAAGGTCTGTGTCCAGGTGACACTGTCTGGAATAATTGACTCGTTGATTTATATCAAATATTAAGCTTTTTGAGTCCTTTAGGTATGCAGCTACCACCTCTCACTTTTCGAGCATTCTGCTGGATAATCAGTGCCTTTCTAATTTCTATTTTCCAA

 

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