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CRIMIC CR70991

Gene AnxB11

CG9968

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:16416958..16422265	14C4-14C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTGTACCTTCTATTTGAGCACCAATC	GTGGTGGTTAGTGGAGGTAGG

Images

Construct Design Sequences

sgRNA sequence	GTTATGATATTTAAGAACCAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGTGCATGTGTGTGAATATTTTCAATTTAACGAAACGCATAATTCCTATTTTGCTCTTTTCCAGTACTGAATCTCAATCACCGGAAATTATCCACAGAATCTCCATAGAACCTCCCTCAAAATGTATCCCTTCGTAAGTTTTGAATGCACATCCTGTAGATTTTAGTTATAAAAGTATCAGCATTAATAAGTGCCTTGG	TTCTTAAATATCATAACATATTCACAATATATTTTATAAATAAGTTTGGTATAACATATTTTTTTGGGTATAAAACTATAATAATTTATTCGGGTGAGTTCTTTCGTTTAGTCTGATTCATTTTTCTAAGAGATAGATGTCATTCTTGTGAGCTCTGTGTTTAATTCAGCGCCATGGAGTCAACTTGAATTGTACATTTT

 

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