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CRIMIC CR70994

Gene Ect3

CG3132

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:11999278..12003516	87A4-87A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGCTGTTTCAGCGAATCG	CTTTGACTCATCATCAGAGCGTTTC

Images

Construct Design Sequences

sgRNA sequence	TCCACATAGCTTGTAGTTAATGG
sgRNA2 sequence	CGCGAGGACCGTTTAGTCGTCGG

 

left_200bp	right_200bp
TTcGTGGTGGACTACGAAAAGGATCAGTTCCTAAAGGATGGAGAACCATTCCGATTCATTGCCGGCTCCTTCCACTACTTCCGTGCTCATCCAGCCACCTGGCAGAGGCATCTTAGGACCATGAGAGCTGCGGGTCTGAATGCGGTGACAACGTGAGTCACTACTATGATTTTAATGGTTCGATTATCATGTTTCCATTA	CGTCGGCCTAAGGACCATTGCGACTTGCATCCATCGCTGTAGCCATAAATCATGCATCATCATCCGTATATGTAGATCTAAATGTAAATTTATGCGGTTTAAGTTCTTTGCAAGACATTAAAGCAACTCCGAGACGGACGCGTCCTGTCTGCCTCGTTATCCCTTTTGTAAAAGACTCATTAAAGCTGTTGTGGCATGCG

 

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