CR70995

CRIMIC CR70995

Gene Ids

CG12014

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:3378268..3380083	63D2-63D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCGTCCGATTTCTCTTCTCC	GCTCGCAGTGGATCGGATTGTATA

Images

Construct Design Sequences

sgRNA sequence	ACGAGCAGTGATCATGTCTACGG
sgRNA2 sequence	TGGAGATGGTGGGCAGCTAGTGG

left_200bp	right_200bp
GCCATATTTTAGATATTTGAATTTTTGAATATATATAGATTACTTGATTTTCAAAATTTTTATAAAACCAAATGTATGAGCCGCTGATATACCCCCTTATCAGATTTCATGGGCACAGGGTAGCACAACAAATTCGCCGGCATCCACATCCGCCGTGTCGGCATCTTCGAGTCAACAGTTGTGAGCTTCCTTCGCCGTAG	TAGTGGAGATCGGCTGTCCCTTCCCCTTCGAATCCCTCTGATTTTGAATGAATTCATAAGCAATAAAGGCAGCGCGACAAACGATCTCGAGACGGCGAAATATCTTGTCGCCATAATTGGAATTTAATACTTCCGGTTGGGCAACAACGTTTGCAAGCCGCGAGACGCCCAGACATGCTGCATATTTTTCGCTGTAATTT