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CRIMIC CR70997

Gene beta-Man

CG12582

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:4197206..4204572	81F6-81F6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAACACCTTCCTGTTTCGAGT	GAAGGCTGACATGCTGTTGG

Images

Construct Design Sequences

sgRNA sequence	AGATTTATTTGTGAGGGCTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTATGAAAGGCAGTAACTACATACCATCCCACTTATTACCCGAAATGCAAACAAATGAACAGAGTAAGTGCACACCATAATTGTAGATTACTTTTGTTATGACTTAGAAGTACATGCTCAAAAAAATCATAGGTAACTTAACTTTAAGATGGGAAACAACAAATAATGTGATTGATGAATCTGATACGGAACTGCCCAAG	CCCTCACAAATAAATCTATTATCTTAAAATTATATATTTTTCAGGTTTTCAATTTATAATATGCTTATAATCAATAGATTTGAGTGATATAATAACTATATAATTAATAATTGTATTTGTATTAAGGCATGCTCTGGTAATCGTAATTTTGTTTTTTTGATTTCGATTTGGGCAAAATCGTTTGATTTCGTTTTTAGGTG

 

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