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CRIMIC CR70999

Gene Lip4

CG6113

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:10527572..10531778	31F5-31F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGAGGGTATATCATTATCACTCGGAG	CAGGGTGAGGATGTATCCATCATC

Images

Construct Design Sequences

sgRNA sequence	TTACGATATTACGTTTGTATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGTTGGAGAACGATGTGGATCCCAATATTCAGGAGGATTCACACTTGAATACCGTAAGTTAATACCCATAATCaGAAAAGGTTAACGCCAATAAAAGATCTAGTAGTAATAGTACATATTTGCTGCTAAAATGTGTTAATGTATACGTTTAAAAGTTAGTCAGATGTTGTCATCGCTTTATTTTTACGATATTACGTTTG	TATAGGAAGCTTTTTGATTTTCGTACCTCTCCCTAATTATTAAGAAAAGTAATTACATTATTGAAAATTGACATATCAATTTAATCGTTAAATTAACGATTACGCTGTTAGAAACTTACACTTAGAAAATTTCTTGTTCTTAGTACAATTGTAATAACCCCGTCTCTTTTCCCTTCTGCAGTACAGTCTGATAAAAAAGT

 

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