CR71000

CRIMIC CR71000

Gene cathD

CG1548

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:7815339..7823569	43E18-43E		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGTAGTCATGATACAAGCTCTTCC	CGAAATATAGTCGTAAGCCTCAAGTGC

Images

Construct Design Sequences

sgRNA sequence	ATTTCTATGTTACGCTTCTCTGG
sgRNA2 sequence	TTTGTTCGATTATTTGGCATCGG

left_200bp	right_200bp
TATATACATAAACGCGAAATATTAAAAAGAAACATTCCGAAAATTAAAGAATTGCATTAGTTTACAACAAAAACTATCGAATCTCTCTAATCAAAACGACGCGCCAAGCCAAATTTAGTACATTATCTGTGCTGAATCATTTTCGCTGTGCCGCAATATTTAATTAACTGCAGCGATCATTGCAGTTTTAGCCACCAGAG	CCAAATAATCGAACAAAGAAAAGTATACTTAAAATTTAAATTGTAAACATTATCGATCGATCCCATTGCGGGTTTCTCCGAAGAATAATATATAAATTATCATTTTAGTAGATTTTCCATATATTTATTAGCGTAAGTTTATTAATTACAATAGGGCTTGCGCGGTCTGCACTTAATGAGACGAATAGTAAGTACGTGGC