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CRIMIC CR71001

Gene tll

CG1378

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:30852315..30854400	100A6-100A

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTCCACATCGGAGTAACCAAGGA	CCGACCTCAAAGCACTTCC

Images

Construct Design Sequences

sgRNA sequence	GGGAAAGTTGGTATAAGTAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTCTACAAAAATCGCCAAAATTACGCCCCACATCGGTATGCAGTCGTCGGAGGGTTCACCAGACATGATGGATCAGAAATACAACAGCGTGCGCCTTTCGCCAGCGGCATCGAGTAAGTATAGCAAAAAAAAAAATCAGATAGCAATGAAAAAATAAGAAAACAATCTTCCTAATATATAAAATTAAGACAATCCTTTA	CTTATACCAACTTTCCCCCCTTCTTGAATTTCCAGGTCGCATTCTATACCATGTGCCCTGCAAAGTCTGCAGAGATCACAGCTCCGGCAAGCATTACGGCATCTACGCCTGTGATGGCTGCGCCGGATTCTTCAAGAGGAGCATTCGGAGATCCCGGCAGTATGTGTGCAAGTCGCAGAAGCAGGGACTCTGTGTGGTGG

 

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