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CRIMIC CR71002

Gene PDZ-GEF

CG9491

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:6323767..6332789

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAAATTTCAGTTCTTTAGACGTACGG	TTTAGTTGGGTCTGCTGAGAGGGT

Images

Construct Design Sequences

sgRNA sequence	ACAGCAAAAGCTACCCCTCATGG
sgRNA2 sequence	GATACGTTGTTTCTGATTGCTGG

 

left_200bp	right_200bp
AATAAGAGGTTTATAATGAACAGAAGCGTATTGAATCCTTTACTTATAAGATGACTTACACACTAATTCTTTTGTATCTAACCATAGTCCTTTCTCAACCTTTTCAGGTAAGTATAAAGTCTAACCTCATCACATATCACATCAAACGGGGCAAAGGTTGGCATGTCGTTGTAGACAGCATCCACAGCAAAAGCTACCCC	ATCAGAAACAACGTATCCCCGATCACCTTTACCCTTTACGGAGCTGCAAAAATATTACTAGCATCCATTTGTATTGCGTATTTAGATTTAGTAACTGAGAATTTAATTTTAGTTAGGGCTTACCATTTATTATTTAATTTAGCTAAATTCTTTGTACACACTTTCTACCCATCATTCACCGCCAGCGTAGAAGTTGCCTT

 

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