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CRIMIC CR71005

Gene Grip91

CG10988

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:13736570..13744683	12B4-12B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCATTGGCCAACAGTTGTTGTC	GTGTCTAGTGTATGTGTTGCATTACG

Images

Construct Design Sequences

sgRNA sequence	GGAAGAAGGCCTCCACCCTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGGACAGGATCGCCGGCATCGATGTGGCAACCAATTCCACTGATATATCGAATATCATTAACGAGATGATCATCTGCATCAAGGGCAAGCAGATGCCCGAAGTTCACGGTGAGATTTGGGAATGTGCAATCACTCACTGGCTGTCCAGGCACGCAAGGCATACGGACTGCTCTGGCATTCCGGGAAGAAGGCCTCCACC	CTTGGGAATCGACCACGTGCTTCAGGATTATAGTTAATCCCAGAAACTCAACATATTTCATGGCATTTTCAAACAGAGAGTCCTTGAGATATGCAACTAACAATACATATACTTATATTTTTATAGTTCTAGATTAAATGTAGCAATAACCATTGCCAAAGTGTGTTGCATAGTCGTAAATGTTCAAAATTAATGTATAT

 

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