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CRIMIC CR71008

Gene Gp210

CG7897

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:5760862..5774324	41F9-41F10		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGACACCAAACAAAGCATCGAG	CCAATAATGTTAGTGCGTAAAACCTGAAC

Images

Construct Design Sequences

sgRNA sequence	AAATGTCATCTTAGCGATATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTAATAGTAAGTGTGCTTAGATGCGTATGAAATGTAAATTACTTGTGGGAGCTTATGCAGATCGAAATAATGCCTTTACTACCCAAGTTTCTTTTAAATATCTGCCTGCACCACCCTATACCCAGCAAACACCACAATAATGATACACACTTGTCTGACCAATATCTAAAGATATTCTTTTATCGACCACATCCCGATA	TCGCTAAGATGACATTTAATGATAATACGTGTCACTCCGTAAAAACCTTAAGATCTCAAACAGAGAAGAAAAGTCAATAATTTAAAACTCCTCCATTTTTATATTTGATTAGTTGGCAGTATCTATCGAATAACCTTTAAAATAAATTGCAATACATTTTTGTGAAAAATTTAAATTCATTTATGAAAATCGTTTTATGT

 

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