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CRIMIC CR71009

Gene Alg11

CG11306

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:21625806..21628571	78E1-78E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAACGCGTCTTGTGGTG	GAACTTCACATTGTCACTATCGACGG

Images

Construct Design Sequences

sgRNA sequence	TTACATACATGGATACCCAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACAGGTAATATTGGAAAAACTGCAAAACCTACATTCACATGCATTCATACGTGTCTTGTTGATCGATCACCAGCTGAAAGAATAGTTATCATTAGAATGAGTTAATTACCTTCGGCTAAAAACTAGAAGAAATGACATATAATAATGCATGGGAGTAGTTCCTCAACAGGGTGCTAACTTTTCATTCTGTTTGCCTTTG	GGTATCCATGTATGTAAATTACATGTGAATACTGTAGCTAGAGAGCCAATTATTTTGATCTACTTGTTTTGTATTTTCTGGCCACTTTACTAATCCTTTATATTTGATATTGTAATTAAAAGGAAAAGTACCAGAACGCCAGGATGGTAATCTACACAGGCGACATAGACGCTTCTCCCAATTCCATACTGCAAAAGGCC

 

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