CR71010

CRIMIC CR71010

Gene dpy

CG33196

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:4477462..4614300	CR71010		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGTTTTGTTTGTGCGTGCCCAAC	GTTTCCTCTGCTTCGCGTTTG

Images

Construct Design Sequences

sgRNA sequence	GGCCAGCGGCAGTTAGCGCAGGG
sgRNA2 sequence

left_200bp	right_200bp
GCGATTGTTTCTCTTTGATCTCTTTGCAGCAAAAATGAAAATATTTTTGCCACTAGTCACGTGGATAGTGCTACTACTCTCGAGTGCAGTAAGTAACCCATAAACATCATTTGTTATTAAGCTAATTTGTGTTAAGTTGACGGGCTAAAACCTGAATATCGATGGGATTAGATCAGAGGCAGAGGCCAGCGGCAGTTAGC	GCAGGGGTCATAATTAATTTATGGGTTCTCGAGAGAGGCCCAAACAAAAGGCCTTACGCACCGTCGGTGAAAAGAAAAGAAAAATGATTAAATTTACGAGCTGCCACCTCATTATGAAACAAATTTCTGAGCAAATAAGCCAAGAGAGATCACAGAAGTGAAAGAACTCCAATGATTAACAAATGTTATTTTGAAGCGAT