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CRIMIC CR71011

Gene Npc2b

CG3153

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:14089517..14092425	88A8-88A8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAATTTAGTTGACCAGCAATTAGC	GCTGAGCGAAAGAAGATAAATCGG

Images

Construct Design Sequences

sgRNA sequence	TCGATGAACTATAATAAAATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCGTCTTACTTTTCTTACAATTAGCCACAACAACATCATCAGAATGAAGCTGAGCTTAGGATTATTTGTGATCTTCGCCGCTTTGATCGGCTTCACGTCGTCCACGGATGTGAGTCAGTGCCCGAAATGTAAGTAAAAACTAGATAAATTTAAAATTAAAAGCAAACAGCACAATTAAAATGTCGATGAACTATAATAA	AATAGGCCACACTCTTGCCTTAATTAACTTGAAGCTTTGAAAGTGAATAGATGTTTAATTAGAGATTAATCTTTTTTAAGATAAGCTCTGTCGTAAATGAATAATAATAACATTTTAACGTTGTGGGTTATAAATGTATTTTGCTTATTACTTCAGCTTACTTACTATATTTTTAGGAATCCAATTTACACTTAACTAGG

 

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