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CRIMIC CR71014

Gene CG17593

CG17593

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:3514872..3517264	23F6-23F6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTTTGCCGAATTCGACGACG	GTTATGAGGGTTCCGACAAAGTG

Images

Construct Design Sequences

sgRNA sequence	AAAGCCTAGTTACATTTCACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGGATCCAGCCACGGCGAGTCCCGCGACGATCCGCCTGCCAAGGAGCCGCGGAAGCAAGCACAAAACCAGGAGAGCGACGAGGAGGACGGCCTTGTCGAGGATGACGATGAGTTTGAGCACTTCCAGGTAAACAAAGTCGGCTGTTCGATGAGAAGAGGTCAAATGCGGTTAGGAACTTTGATCAAGAATATGCCCAGTG	AAATGTAACTAGGCTTTCCTATAGGTGGCCTTGTGTTCCTTTATTTCCTTCATAAAATACTTGTTCATAAGGGTACATAAAGAACCATTGGGAACATTTAATACATTGCTTTTAAACAGCTTTCACCATGATGACATGGAATTTTGCCTTACCTTACTTTAAATCTTATTTAATGTGAAGTGCTTCTACAAATCTGTAAT

 

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