CR71015

CRIMIC CR71015

Gene Arf102F

CG11027

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:1124167..1125325	102F8-102F		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCGCAGTCAAAACATTGTATTATCG	ATTCTGGTAATGGTATTGAGGCATAC

Images

Construct Design Sequences

sgRNA sequence	AAATAGTTTCTTATTAAACATGG
sgRNA2 sequence	TTCATAAAGACCGTGCCCTTGGG

left_200bp	right_200bp
TTATTGTTTTCATCAGACCATGCTTAGGGTATAGTCAACATACTGTTATTAGTATTTTATAGAAAACAGTATTTTTAACCATCGTTTAACTTTTCTGAAAAGTACAGCATTATTAATCGAATGACTAGTTTACACGTCAATTTACTGAAGTTACTGAAGACAAGCTTTTTCCTATACGAAATAAAATAGTTTCTTATTAA	GGCACGGTCTTTATGAAGGACTTGATTGGCTATCAGCTGAATTGGCTAAAAAATAAAAATATTCCTTATATTGTAAGATAGGAATTGCTGTTCTCGTATGCTGAAAACTAGATTTTAAATAAACTTGTAAATGTATATAAACTCGTTTGGAACTTTGTTGTAAATTTAAAATGTCCACGTTTTATGTGTTGGTGAGAAAA