CR71016

CRIMIC CR71016

Gene Rab5

CG3664

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:2359142..2365273	22E1-22E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TGGGCAGCATGTTTTCAAGGG	TGCTCAGAATCTTACACGACATGTA

Images

Construct Design Sequences

sgRNA sequence	ATCAGTTGCCATCTCAGAGGAGG
sgRNA2 sequence	ACTTTCGTTTATGTTGGGAATGG

left_200bp	right_200bp
AAAACGTACCATTATGGCAGAAGAATTGGAAGCACTCAATTTCGTTTTTATCCGTCTCCATTATAAATATGTACATTATTTGTATTTTGCAGATTCCGCATCCACACTCAGCAGCAATCTTAGCAGAAAGACTTACTCAGCAGAAGAGGGAGTGAGTTGCAGTAACCGTCCGTAGAACCAAATATCAGTTGCCATCTCAG	CCAACATAAACGAAAGTTGCAATTAAAGAAAGTTAGTTACAAATTCCCACACCCGATTTCCCCCATTACGCTCGATATCTGTCCAAAAAATATACAAGCAAACTGACACTCACGCAATTGAGCTAAAATATCAGTATAATTCCAACATAAACCATTTCAAATAACAACGCAAATTAATAATGAAAACTAAACATGAGAAG