CR71021

CRIMIC CR71021

Gene GC

CG13927

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:1623179..1629301	62A9-62A9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GATGTCCAATCTGAGTTACCACTG	CACCCATACATTCGGTTATATCGCTC

Images

Construct Design Sequences

sgRNA sequence	ACCCAGGGAAACATGCCTGATGG
sgRNA2 sequence	TTTAATATCGAATCTAGATACGG

left_200bp	right_200bp
TCGTGCATTGGTTCACCGCCTTCTTCGACGTGTCCATTGCTTTTTTCATGACTCTGGAAAAGACGCGCCTACTGGTCACTCCTTTCATGCTCAGTTTTCATCTAATGAACTCCCGACTGTTTGTAATAGGTAAGTGTCCTGTTAACTATTTATAATCCTACAAATCAATAATTATGTAATTTTGTGTAACTTACCCATCA	CTAGATTCGATATTAAATCTGGTTAAGGAAATTCAAAACCTATATTGCCTATAAATCAGAGGATAACAATTTATATTGAAGAAATGAAGGTTCGATTTGAAGGATATACACAAATCCTCTTGGTTTTGTCTATATATCAATGAAATGCATTTAATAATATCACATTATTTTGCATTTACATAACGATACGCTCAAATCAA