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CRIMIC CR71022

Gene mRpS25

CG14413

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:14582136..14582982	12F1-12F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTACACAAGCAGCCGCTC	GCGCTACGGAAAGTCATCG

Images

Construct Design Sequences

sgRNA sequence	TAATAAATGCAATGCGCACAGGG
sgRNA2 sequence	ATCTTGCCGCGCATGTGATCGGG

 

left_200bp	right_200bp
CAAAATGCCGTTCATGAAGGGCCGTGAGCCCATCCGCCGCACCCTGAAGTATCTGAATGCCGGTAAGCTGGTGCTGAAGGACAAGGTGCGCATTTTCAGCGTGAACTACAACACATATGGTGCTCATCACGCGGGCGCTCGGTAAGTTATGGCATAGAATTGTCTAATAACTGAAATATACGCTAATAAATGCAATGCGC	CACATGCGCGGCAAGATCTTGTTCGCACCCAAATAGACGTTCGCTCTGATTGTACCTTTTTTTTTAATAAATTAATTGTAAATTACACCTGCAAGGCTCGTCTGCTGAGGTAGCGCCCTAAGTACTAATGATTTTTACAGACTAATGCGTGAGACGGTGGCAAAGTCGAGTGTGGGTCGATTGAGCCTAGTGGCGCTTGG

 

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