CR71030

CRIMIC CR71030

Gene Usp5

CG12082

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:3145405..3148358	63B6-63B7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAATTGTGCTTATTGGGCGGC	GCATTATTAGAGATCTTGTTCCTATCTCCAG

Images

Construct Design Sequences

sgRNA sequence	GATTCCAGATACCGTTATGGAGG
sgRNA2 sequence	CTTGTGGGACTGGGGTGATTCGG

left_200bp	right_200bp
GAATTGTTAAAAGCTGATTTCAACCAGTAAAACACCATTTCAACCCATCGATATTCGCATATCCATGCTCGCCGGTATCGATGCATTTTCCCATGTTCATCTCTAAGCAGCAGCTGCAAAAAAAATCAACTATACTATTTTACATTCGGTCCGTAAAGTTTATGAGAAAAGAGCTGCCTACAGGATTCCAGATACCGTTA	CACCCCAGTCCCACAAGCTAATATTATTCGCCCTGAAGAATGGAATTTAATAATAAAGTACATAAACAGTGCTGCAATCGGTTTTTGGGGGCTTCTGATATATGCAATCTTCACATTGATGAATCATTTGATGACTAATATTTCGCATAAGCAGCTAAAAATAATTCCATAAGAGTTTACAATAGATCTGCTTTCTTAAT