CR71031

CRIMIC CR71031

Gene CG9014

CG9014

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:13722161..13723642	34D1-34D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCATAACAGATGATCGAAAGGCCT	CAATTGCAGGCTAAAATCGTAATGGTC

Images

Construct Design Sequences

sgRNA sequence	GAAATCAAACCATTTCGTCATGG
sgRNA2 sequence	ATAGAAGCTCAGTGAACACTCGG

left_200bp	right_200bp
ATGCCAGCTCGGACACAACTCAGCGCCGACGTAAAAACTAACTTTTTGATCGAAATATTGTAAAAATCGAGTTTCGGATATAGGTATAAGTACATATCGAACTCAGTGGTAAGTGGATGAGAGTGGCTCCATGCCAAAAAAAAATAACATCTCAGATTCTCCAGCGACATAAACTTCGGTATAGAAATCAAACCATTTCG	ACTCGGGCGTATGCGTAATGTTGGAGCGAAATTAAATAAACGCATAAGCTGGGATTGAGGTTGCTCCTTCTGTGGGTGGGCGGTTGGTTGGGCTTGCGGTGGGTGCATCAAGTAAAACTTATAATGGAAATGGTAAAAGTTTGGCTGTGCTCGGTTCGCGGCAGTTGGCGACACCATTTGGAAAAGTTTTACAAATTGTA