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CRIMIC CR71032

Gene Tim9b

CG17767

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:19616663..19618537	18D3-18D7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCATTCAAAAGTACTGCTCCATTCC	AGCCTGTTGACCCAGCAG

Images

Construct Design Sequences

sgRNA sequence	TCCGCTCGTCACGAACGTGACGG
sgRNA2 sequence	ATCTGTCCATGTAGCAGCAGTGG

 

left_200bp	right_200bp
GTCCTATTTTACTTTCTGCCCCCCTGGGGAGATGAACACTTTAGTTGCTCTCAAATAAAAAGTAAAAACGAACACAATGCGAAAAATTTGGGTATCTGTATTTTAAACTCGATAACGCACACTGATTAGTCTGCTTGCCGTGCTATCGACTATCGTTTTACCAAGCACAGACTAATCAGCTGTTCCGCTCGTCACGAACG	CAGTGGCATGCAACGCCGCAGCTGCTGTTCTCGATCCTAGATAGCTAATCGCAGAGTCCAGACCGCCCTCCACACCCGCAGTCGGCGACCACACGTACTCTTCCTACAAACAACCCCAAAACAAAAAAAAAAAAAAAACGAACCAACCAACCACACGCGATGCGGCGCATTTGTCTTGTTGCACTTATCGGCCTGCCAGC

 

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