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CRIMIC CR71034

Gene Fas3

CG5803

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:18320102..18393441	36F2-36F4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTTTTATATGTCTCGCAGCCATC	AGTGTAATTGACCTTTTCAACCTGTG

Images

Construct Design Sequences

sgRNA sequence	AACACTTTCCACAGCCCCGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAGCCAAAGTCGCGCTCATGAGATGATATCACGCGCTCGTTTAAGACCCGCGCAAATGTGTGAAAAAGTTTAGCTTACTCTCAGATACCTATGTATATGAATAAAAAGATGCTACCCAAAAACGTGTTCTCACCTCGAGCTGTATAAAAATATCATTGTTGAACGGCAAACTTGATTGATGAGAAGACATCGCCCCGCG	GGGCTGTGGAAAGTGTTGTCTTTATGAAAGTGATGATTGAGTGAAAAGTTCCCTGTCATAATATCCATGAAATTGATAGTTACTCCTCTTTTGGCGGTTGACCCCGTTTGGTCCATAGATCCAACTGCGCATGCTCAGAGTGTGACCGAAGCTGTTTTGAATCTGTGTCCGTGCCAAGTGTGACCTTTCGCATCTTGGGG

 

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