CR71036

CRIMIC CR71036

Gene mlt

CG12214

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:10155916..10158931	46F3-46F3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTTTGGGTTTCTTTGTTACG	GTGTGTGGCTTAGGTTAGGCAG

Images

Construct Design Sequences

sgRNA sequence	AGTAAAGCCCGAATAAATATAGG
sgRNA2 sequence	AGAAGTGAGGCGCGACGTATGGG

left_200bp	right_200bp
GTGCGCAATATTTAACGGAATAACAAGAAACACAACGAACGCGAAACGTGCGCAATAATTTGTTTAAAATCAATGAAGACTGCAACTGCGCAAAACTGAAAAATTGCATCATAGCCAACACAGAACGGGGCAGGGAAAAGTCTATGAGCGAGGAAAAGTGGAAAATCAATAGGGGGCAGAATAAGTAAAGCCCGAATAAA	TATGCGCTACATGCTTCCGTATCTGGAGAGGATTACCATTAACCCGAACCGCGCTTCGTCCCCCGCCTGGTGCTACAACTTGTGATCTTGTACACGAATCATTTACATTTACTTTACTCTACTGTTAGTGCCGTTCTGTTCTCCGCAGCGAACCCCTGTGTACATACGTTCTGAAAATGGCCAAGCGAGCATTACTGCGC