CR71040

CRIMIC CR71040

Gene Yod1

CG4603

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:5133871..5135670	64C7-64C7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGTGCTGCCACTCTTATC	GTGAATTCAGGACAGCTTTCTGG

Images

Construct Design Sequences

sgRNA sequence	CTCTTCGTGTGGGCCTGCGATGG
sgRNA2 sequence	CTCGCACGGCGATTGTGGCTAGG

left_200bp	right_200bp
CCAACAACATTACTCACTGCACTAATCGATTATAATTATATATTAAAGCACAAAAAATACCAACAGATATCGAAATTCGATAGCATCGATACGCTGTAACAAACAAATAAAAAAAAAGAAACAAATTAGTCAAATTACAAAATTTATCAGTTATCCAATTGCACACAAAAACCAACTCAAAATTAGCCCAATTTCCATCG	CACAATCGCCGTGCGAGGAGTCATCTCATTAGCAGTACTACCTCCACATCACCGCCTACCTAAGTTATTGATTATTGAATTGAGCCCAGCCGCCTGTATTCATGTTAGTCCCCTTTTAATCCCTTTCGGAATGCAATAAAATATACCTTCATCTACACTAGGCAAGTCTTGGAGTTGGTACCTATATAGCTATAAGGTCA