CR71041

CRIMIC CR71041

Gene CG5245

CG5245

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:12429560..12431235	87B10-87B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CGAACCATATTCCTGTGGGGATGA	TTGTACTTATTGCCCAGTCATGG

Images

Construct Design Sequences

sgRNA sequence	CAAAATTCCGGTGCTAAAATCGG
sgRNA2 sequence	GATCAGGGGGACTAAACAAATGG

left_200bp	right_200bp
TAAACATTTTACTACATTAGAATGCATAAATTGATTGAATTAAAATACGGAGACTGGTGTTTTTAATCTGCATAATTTTGTAGTTGTAGCTAGTGCAACAAGGTTTCTATCAGTTCCAGGAAAACGCTTTGTTTACAAGCAAAGTGCTGATTTTTATGTTGATGGTCTTACCCTTTTGTTGTACAAAATTCCGGTGCTAA	GTTTAGTCCCCCTGATCGACCTGTACCATATTTGGCATTCAGCATTCGGTCACACTGCCGCCCAATTGCTGTGTCATTTTTGTTTCTGTGCGAAATAAGTCGGCTTTTTCATTTTACACATCAAATCACTGCATTTGCGGCCCAGGAAATATGGCTACCGAACGATACAGCTTTTCGTTGACCACGTTCAGGTGAGAGCA