CR71042

CRIMIC CR71042

Gene Iru

CG11982

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:9006456..9008030	85C2-85C2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAAAAATATCTCCGCGAATG	CTGTATAGAACTGGCAACGCCTAC

Images

Construct Design Sequences

sgRNA sequence	CCCAATGACCGGGTAAAGCTAGG
sgRNA2 sequence	AGGGCGATGCTAAATCGAAGTGG

left_200bp	right_200bp
TGGATCCTTTTGAAGTAATAAAAGATCCAAATGTTAATATGCCAGTATTAGCTTTCAGTGTGACCAGGCCGCGTCAATCAGTTGGGATTTATTCTCCTCTGCGGCAAAGGCCACTTTGTAAGGGGCCCTTATTTATAGAAAAATTAAATTCTATCCCTTAAACTCCCAATTGTTCCGACTGAACCCAATGACCGGGTAAA	AAGTGGAGCGATGATCGATTGATATTCAAAATTAAGCCAAATATTGGCCGCATATATATTTATAATAGTTTCTCCTCACGCTTAATTTTGTTGTTGTTCAAAATAACGATTTGTAATAAACAAAACGGGACATACGATTCCGTTGGCTCTGCAAAAATCAACTCTCGTTTATCGTATCGCGTAGTTGCTTATTTTTCTTT