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CRIMIC CR71043

Gene CG11459

CG11459

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:6025826..6027238	83D2-83D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCGTGATTCTTGATTAGTTCATATGAG	CTTCGGCTTGGCGAAGC

Images

Construct Design Sequences

sgRNA sequence	GGCTGCGAATGCATTATCTGGGG
sgRNA2 sequence	TAGAGAAATATGATGTCAGTAGG

 

left_200bp	right_200bp
AGATAAATTTTCAGTAAGTAAACTTAGCAAGCATTATAGCTAATATAATTATAGCTATTAGAAGTAACTTACAACCAGCCTATAACCAGAAACCATATAAATAAATAATATAATTTTATACATAAGCAAAATATTGCATTTTAATCGTAATCTTTTCCAATTTCCCAAAACCAATAAGCAGTTCACATAAATAACGCCAGATAATGCATTCGCAGCCGCCAGGCAAACATAAAGCTCTTGATTTCTAGTTTTTCCAGCTCAAAATCATTTACAAATCTACTTTAAACGCGATCAGTTCGCA	AGTAGGATCATATCTCCCAGTTGAAATAAACGTATTTTATATCGCTTTTGCATTAATGTTTATTCACATTAGCTAAAATTGTAGATTACATAATCATAAGGGTTTCTCGGAAGGGTCAGCACGTCAAATGCGCTTACTGAGAAGCATTTCTACCAAGAAAGCCCAGAGTTTAAGGACTCTGTCAGAGCAACTTGCCTCTT

 

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