CR71044

CRIMIC CR71044

Gene CG4593

CG4593

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:7013887..7015009	6E4-6E4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCAAAAAGCAAACAATAACTGTTTGAC	CCAGTCTAACGCACTAAAACCGTC

Images

Construct Design Sequences

sgRNA sequence	TTTCTTGGGAGGCTTGATTCAGG
sgRNA2 sequence	CATGTAGTCCCCGGGTGGGCAGG

left_200bp	right_200bp
ACAACTCTGATTTAATATAAGTAGACGAATTTGTGAAAACGGTGCCCTAAAGAAATTTGAGTTATTAAAGCCAAATTAAAAGCGGAATTTATATATATACTAAGTGATACAGAAATCAGCACTATGCAAATTCCACTGTATTTCATTTGTATTTCAGACAAGACGTCGTCTGGTCACACTGAGCCAGAGGTGGACCTGAA	GGCAGGACCAAGTCCTGGTGCACCCTCGCATTTACCCAAAACATCCTTTGCCATCCTCGACCTTTCTTACTCTATCAATCGCACATCATTTTGTAAATGCCATCTTTTTGGTTCATTAGAACGTTTGCGGCAGGCATTGCCAAAGAATTGCAGTGTGAGAAAAAAAAAATATAGATTGTAAATATAATAATAATAATAAC