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CRIMIC CR71045

Gene Polr2E

CG11979

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:10898031..10899060	47C6-47C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCTGCCGCGTTAAAAGG	TGGATTAGGATTTGCTGCTCAG

Images

Construct Design Sequences

sgRNA sequence	TAAATTATAAACAGAGCACGCGG
sgRNA2 sequence	TTTAAGAAACCTATGATTTAAGG

 

left_200bp	right_200bp
GCTCCGTACAGATCGTACAGGGTGCTCCGTACTTGAACAGTGGAACAGAACGGACGGCTGCTACGCTCTTATAAATAGGTTCGAGTATTCGCTAAGCATGTGACCATCAAACTCTCTTTGGTATTTTTAATTGTTATGCTGCAGGCGCTGTAAGGTATTTTATAAAGCGGTATACACTGGTCTTACCACATCATCCGCGT	TTAAGGGAAAGGCAGCGAGTTGTACACTTTAATAAATCGCAATCTTTTCAGTGAGTTGTGTGTTGGGTATTGGTAAGAAATTAAAGTACTCGAAATTTAATCCCCTTGAATTCTAGGGACTAGTTCAAAGTCTCAGCATTTTACTTAAGAGAATCAATAGTACAAGTTGACAAAATGATATAAATTTAAAATAGTGTATT

 

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