CR71046

CRIMIC CR71046

Gene CG15403

CG15403

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:3128635..3129489	23D2-23D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGAACTGTTTATGCTAGCGCGAG	AAGAACCCCGGATGAAGATG

Images

Construct Design Sequences

sgRNA sequence	CTTCGTTCATAAGTCATTCAGGG
sgRNA2 sequence	AATTGAAAGCGTAGATCTGCAGG

left_200bp	right_200bp
TAATGAATTTATATCATTACGGGGGCATTACTACACAGCCAGCTAATTATGCACGTACACGTTAGGAAAAGCGGGAGAAAATTATATCAAGGGGAAAACTTTATGCAACGTGATAGATAAGAGGGAATTTTGACATTACATTTCGATTACGATTGAAACTAAAATACTCGTCATCATGTACTACTTCGTTCATAAGTCAT	TGCAGGTCTAGAGCTAATTCCATTTTGTATTCCACTATGTAAATGGCAAATAGAAAGAACTTTTTGGAAAACCAATTTATATTCGCTCCATATCATCGACACCATCGGCCTGAAAAGTTAGTTTAATGTGCTAAATGCTTGCCAAACAATATCCGACCGCAAACTTTGCCAACGTTTAGACTGACAAGGAAAAGTTTCTT