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CRIMIC CR71048

Gene Atg4a

CG4428

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:1080911..1085380	21E4-21E4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAGTAGATGCTGGTTGG	CTCTGTGTGTGTGTGTTTTGCC

Images

Construct Design Sequences

sgRNA sequence	TGGTCAGCATGAGTCATAAGGGG
sgRNA2 sequence	TTGGGTGCTCCACTTTCGCCTGG

 

left_200bp	right_200bp
AGGCGTACTTGGGACCAGACAGCGTCCTCGCTAGTGCAGTTGGCCAGGCGGTGGGCAGCGGAGAGCCGGAGGACATTCCCCGAAGAAACACCGACGTCTGGGTCCTGGGAAAGAAGTACAACGCCATACAGGGTAAGATGAGACCTTATTTAATGCTGTCATATGGGTACAAATCTAGCATATTGGTCAGCATGAGTCAT	GAAAGTGGAGCACCCAATGAGGAGAAAGAGAGTGGGCCGGGAGGTCGAGCCCCTGCGGATGCTGCGAATGCAATAAACTAAGGCTAAAGTGCATAATTAGACGAGTGGTTGAGATTTAGATTTTAGTATGTTTACGATTTGTACGAATGCGGTTCTTAGCGATTCATAATGCTGCTTTATTGTACGCGATTGACTCCACG

 

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