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CRIMIC CR71049

Gene Atg4b

CG6194

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:15353720..15356924	88F1-88F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCCTCTGTTTCCTATTTGC	CATACACAGGATTCAGTTTATCACTGC

Images

Construct Design Sequences

sgRNA sequence	TTCGCGAAGCTCATTACTCAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTTCACCACAGACACGCCTTAGAGCACGCGGCTCAGGAAAATGCAGACTTTGACAATATTAGTGTCTATGTAGCCAAAGATTGCACGAGTAAGTTCAAGCAGAAAGGAATCCCTTAATCAAAGATTCATAAGATAACTACCATGACACATTGATATGTTCATGAGTAATTGCTGCAACCTTGTTCGCGAAGCTCATTAC	TCAGGGGCTCAATTGATAACGTATGTGATTTGATAACCGTCTTTGTGTTGTTTTAGTTTACTTGCAAGATATTGAGGATCAATGCAGCATTCCGGAACCGGCGCCAAAACCCCATGTGCCTTGGCAACAAGCGAAGCGGCCGCAGGCGGAAACTACCAAAACGGAGCAACAACAGCACTGGAAGTCCCTCATCGTTCTGA

 

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