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CRIMIC CR71052

Gene Pp2A-29B

CG17291

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:8366068..8370090	29B2-29B2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATTATGGGTATACGTCTTTATCCGC	CAGCAGTACCTCGTCCTCATC

Images

Construct Design Sequences

sgRNA sequence	GTTATTATTGATAGGGCTATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGATCAACTTCAATCAATAGTAATACCATAACTGATTTTAAAATGAGATAGTAAGATGAAATATTTTCGTTACATCGAATAATCTAATCACTTATCTTATCTAAAAAAGATATCTTAAGTAAATATATATGCTACGTTTTTTTTTGAAAATAAAATGTAGGGTACAGGTTGACCACCGCTTTTGTAAAAAAAAACCAATA	GCCCTATCAATAATAACCTCAAAATCGTAGAATTAGTTACCACTTATCCTAAATTTGAATACCCTATTAAAATAATCATCTTTAATATTTTCCCTTACCAAAACAGCTTCGGTTGAACTCCATCAAGAAACTGTCCACCATTGCACTCGCTTTGGGCGAGGAGCGCACACGGTCCGAGTTGATTCCCTTCCTCACCGAGA

 

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