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CRIMIC CR71055

Gene CG31712

CG31712

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:9934767..9938134	30F1-30F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGGACGACATCGCGAAC	CTCCTGTTCGATTTCGTCGC

Images

Construct Design Sequences

sgRNA sequence	TGTCATCTTGAGCACTCCATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACACCGCAGCGACCGACACACTGAGCGCGACTACCGTCACTCTCCCTCGATCCTGAAATCCCGAAAACGCTCCAGCTCGTCGAGCAGCGACAGTCAGTACTCCGAGCAGGAGTCGCAACGCAGCAAACAGAAGCGCAGCCGCTTTAAGAAGCTGGATGAGGTAAGTGGATAGTGTCCATAGTCTGTCATCTTGAGCACTC	CATGGGTACGCATTCCATACATGCATTTTTCCGGGATGAACACTCATGCTGCGCTTATGATACAACTGTTTCGATGAAAGCAACTAAACTATGATCTACCATTACAGCAAAACCAGATGCAGGTCGAACGCCTGGCCGAGATGGAGCGGCAGCGTCGGGCGAAGGAGCTCGAGCAAAAGACAATCGAAGAGGAAGCCGCC

 

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