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CRIMIC CR71056

Gene CG10588

CG10588

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:20956934..20960660	78A1-78A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCTGCAGGGCATGATTG	CTTTGGCATTGGATGTGCTAGC

Images

Construct Design Sequences

sgRNA sequence	TATTCGATGTACATTATGAAGGG
sgRNA2 sequence	TTTGATCGGTAAGACTCCACTGG

 

left_200bp	right_200bp
CATGCCGAGCACTGAATGGATTTTAACAACAGCTTCGTTTCTGACCGTATAAAAGTGGCTTTAGGAAAACTAATCGGTGTAAAATAATTTGATTAGAATTACGATTGTATAAATTAATGGCAACAACTCGATGCTCTTCTAACTAGTTAGTGGATTCCCTTAAAATATCTCCGTTCGTATGCTGTCAGAAAAATCCCTTCATAATGTAC	GAGTCTTACCGATCAAAATACGATGTATTTCTATCTCGGTATGCCTATAATTAAGCAAGCCAAATCCGAAAGAGCGAGACCAAATGAGACCAGAACATATTTTATAAATGATTTCTAAGTGTAGAAAGCTTTTATTTATCGTTTTGCTAGCAAAGCTTTAATGCTTTTCTCGATAAAAGGCTTCACGTACATAACATCCG

 

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